학술논문

Genome size evolution in the beetle genus Diabrotica.
Document Type
Article
Source
G3: Genes | Genomes | Genetics. Apr2022, Vol. 12 Issue 4, p1-9. 9p.
Subject
*GENOME size
*BEETLES
*NUCLEOTIDE sequencing
*INVERTED repeats (Genetics)
*FLOW cytometry
CORN disease & pest control
Language
ISSN
2160-1836
Abstract
Diabrocite corn rootworms are one of the most economically significant pests of maize in the United States and Europe and an emerging model for insect-plant interactions. Genome sizes of several species in the genus Diabrotica were estimated using flow cytometry along with that of Acalymma vittatum as an outgroup. Genome sizes ranged between 1.56 and 1.64 gigabase pairs and between 2.26 and 2.59Gb, respectively, for the Diabrotica subgroups fucata and virgifera; the Acalymma vittatum genome size was around 1.65Gb. This result indicated that a substantial increase in genome size occurred in the ancestor of the virgifera group. Further analysis of the fucata group and the virgifera group genome sequencing reads indicated that the genome size difference between the Diabrotica subgroups could be attributed to a higher content of transposable elements, mostly miniature inverted-transposable elements and gypsy-like long terminal repeat retroelements. [ABSTRACT FROM AUTHOR]