학술논문
Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells.
Document Type
Article
Author
Source
Subject
*FLOW cytometry
*CELL culture
*IMMUNOHISTOCHEMISTRY
*MONOCLONAL antibodies
*APOPTOSIS
*CELL receptors
*HEALTH outcome assessment
*DESCRIPTIVE statistics
*CELL lines
*MEMBRANE proteins
*BREAST tumors
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Language
ISSN
2008-2835
Abstract
Background: Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells. Methods: In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines. Results: Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines. Conclusion: Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies. [ABSTRACT FROM AUTHOR]