학술논문
Peptides Derived From Insulin Granule Proteins Are Targeted by CD8+ T Cells Across MHC Class I Restrictions in Humans and NOD Mice.
Document Type
Article
Author
Azoury, Marie Eliane; Tarayrah, Mahmoud; Afonso, Georgia; Pais, Aurore; Colli, Maikel L.; Maillard, Claire; Lavaud, Cassandra; Alexandre-Heymann, Laure; Gonzalez-Duque, Sergio; Verdier, Yann; Vinh, Joelle; Pinto, Sheena; Buus, Soren; Dubois-Laforgue, Danièle; Larger, Etienne; Beressi, Jean-Paul; Bruno, Graziella; Eizirik, Decio L.; You, Sylvaine; Mallone, Roberto
Source
Subject
*T cells
*PEPTIDES
*TYPE 1 diabetes
*T cell receptors
*INSULIN
*PROTEINS
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Language
ISSN
0012-1797
Abstract
The antigenic peptides processed by β-cells and presented through surface HLA class I molecules are poorly characterized. Each HLA variant (e.g., the most common being HLA-A2 and HLA-A3) carries some peptide-binding specificity. Hence, features that, despite these specificities, remain shared across variants may reveal factors favoring β-cell immunogenicity. Building on our previous description of the HLA-A2/A3 peptidome of β-cells, we analyzed the HLA-A3–restricted peptides targeted by circulating CD8+ T cells. Several peptides were recognized by CD8+ T cells within a narrow frequency (1–50/106), which was similar in donors with and without type 1 diabetes and harbored variable effector/memory fractions. These epitopes could be classified as conventional peptides or neoepitopes, generated either via peptide cis-splicing or mRNA splicing (e.g., secretogranin-5 [SCG5]–009). As reported for HLA-A2–restricted peptides, several epitopes originated from β-cell granule proteins (e.g., SCG3, SCG5, and urocortin-3). Similarly, H-2Kd–restricted CD8+ T cells recognizing the murine orthologs of SCG5, urocortin-3, and proconvertase-2 infiltrated the islets of NOD mice and transferred diabetes into NOD/scid recipients. The finding of granule proteins targeted in both humans and NOD mice supports their disease relevance and identifies the insulin granule as a rich source of epitopes, possibly reflecting its impaired processing in type 1 diabetes. [ABSTRACT FROM AUTHOR]