학술논문
The Effects of a Single Freeze-Thaw Cycle on Concentrations of Nutritional, Noncommunicable Disease, and Inflammatory Biomarkers in Serum Samples.
Document Type
Article
Author
Abraham, Ransi Ann; Rana, Garima; Agrawal, Praween K.; Johnston, Robert; Sarna, Avina; Ramesh, Sowmya; Acharya, Rajib; Khan, Nizamuddin; Porwal, Akash; Kurundkar, Sucheta Banerjee; Pandey, Arvind; Pullakhandam, Raghu; Nair, Krishnapillai Madhavan; Kumar, Geeta Trilok; Sachdev, HPS; Kapil, Umesh; Deb, Sila; Wagt, Arjan de; Khera, Ajay; Ramakrishnan, Lakshmy
Source
Subject
*FREEZE-thaw cycles
*NON-communicable diseases
*TRANSFERRIN receptors
*BLOOD sugar
*BIOMARKERS
*FERRITIN
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Language
ISSN
0974-2727
Abstract
Background The stability of biological samples is vital for reliable measurements of biomarkers in large-scale survey settings, which may be affected by freeze-thaw procedures. We examined the effect of a single freeze-thaw cycle on 13 nutritional, noncommunicable diseases (NCD), and inflammatory bioanalytes in serum samples. Method Blood samples were collected from 70 subjects centrifuged after 30 minutes and aliquoted immediately. After a baseline analysis of the analytes, the samples were stored at − 70°C for 1 month and reanalyzed for all the parameters. Mean percentage differences between baseline (fresh blood) and freeze-thaw concentrations were calculated using paired sample t -tests and evaluated according to total allowable error (TEa) limits (desirable bias). Results Freeze-thaw concentrations differed significantly (p < 0.05) from baseline concentrations for soluble transferrin receptor (sTfR) (− 5.49%), vitamin D (− 12.51%), vitamin B12 (− 3.74%), plasma glucose (1.93%), C-reactive protein (CRP) (3.45%), high-density lipoprotein (HDL) (7.98%), and cholesterol (9.76%), but they were within respective TEa limits. Low-density lipoprotein (LDL) (− 0.67%), creatinine (0.94%), albumin (0.87%), total protein (1.00%), ferritin (− 0.58%), and triglycerides (TAG) (2.82%) concentrations remained stable following the freeze-thaw cycle. In conclusion, single freeze-thaw cycle of the biomarkers in serum/plasma samples after storage at − 70°C for 1 month had minimal effect on stability of the studied analytes, and the changes in concentration were within acceptable limit for all analytes. [ABSTRACT FROM AUTHOR]