부산대학교 도서관

Lentil cultivation could be challenged by combined heat and drought stress in semi-arid regions. We used RNA-seq approach to profile transcriptome changes of Lens culinaris exposed to individual and combined heat and drought stresses. It was determined that most of the differentially expressed genes observed in response to combined stress, could not be identified by analysis of transcriptome exposed to corresponding individual stresses. Interestingly, this study results revealed that the expression of ribosome generation and protein biosynthesis and starch degradation pathways related genes were uniquely up-regulated under the combined stress. Although multiple genes related to antioxidant activity were up-regulated in response to all stresses, variation in types and expression levels of these genes under the combined stress were higher than that of individual stresses. Using this comparative approach, for the first time, we reported up-regulation of several TF, CDPK, CYP, and antioxidant genes in response to combined stress in plants. • Comparative transcriptome analysis of lentil under combined heat and drought stress for the first time • Most of the DEGs altered in response to combined stress were not expressed in individual stresses • Total number of DEGs under combined stress was considerably higher than that of individual stresses • Most of the genes were up-regulated in response to combines stress • Up-regulation of several genes in response to combined heat and drought stress in plants for the first time [ABSTRACT FROM AUTHOR]