학술논문
New micro/nanocomposite with peroxidase-like activity in construction of oxidases-based amperometric biosensors for ethanol and glucose analysis.
Document Type
Article
Author
Source
Subject
*GLUCOSE analysis
*GLUCOSE oxidase
*SYNTHETIC enzymes
*BIOSENSORS
*NANOCOMPOSITE materials
*GOLD nanoparticles
*ETHANOL
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Language
ISSN
0003-2670
Abstract
Development of artificial enzymes, including nanozymes as an alternative for non-stable and expensive natural enzymes, is a booming field of modern Biosensorics and Biofuel Technology. In this study, we describe fabrication and characterization of sensitive biosensors for the detection of ethanol and glucose based on new micro/nanocomposite electrodes with peroxidase-like activity (nanozyme) coupled with microbial oxidases: alcohol oxidase (AOX) and glucose oxidase (GOX). The nanozyme was synthesized by modification of carbon microfibers (CF) by hemin (H) and gold (Au) nanoparticles. The formation of gold nanoparticles on the surface of hemin-modified carbon microfibers has been confirmed by the UV–Vis and X-ray spectroscopy as well by the SEM analysis. Compared to hemin-only modified electrodes, the resulting micro/nanocomposite CF-H-Au electrodes exhibit a higher specific catalytic activity and a better affinity for H 2 O 2 in solution. The H 2 O 2 -sensitive CF-H-Au-modified electrodes showed a higher sensitivity (1.3–2.6-fold) compared with the nearest carbon-derived analogs and were used for the construction of highly sensitive ethanol and glucose biosensors. To eliminate diffusion limitation for substrates, AOX or GOX were fixed on the CF-H-Au-modified electrodes using a highly porous Nafion membrane. The main biosensors' characteristics have been investigated. The developed biosensors were tested for ethanol and glucose analysis in the real samples of both grape must and wine. The results are in good agreement with the results obtained using enzymatic kits as reference approaches. Image 1 • A new micro/nanocomposite with peroxidase-like activity was synthesized. • The nanozyme is based on carbon microfibers modified by hemin and gold nanoparticles. • It was used for construction of highly sensitive ethanol and glucose biosensors. • The biosensors were tested for ethanol and glucose analysis in the real beverages. • The obtained results have a high correlation with the reference approaches. [ABSTRACT FROM AUTHOR]