학술논문
In Situ Imaging of Fluorescent Nipah Virus Respiratory and Neurological Tissue Tropism in the Syrian Hamster Model.
Document Type
Journal Article
Author
Source
Subject
*GOLDEN hamster
*NIPAH virus
*DELAYED onset of disease
*CENTRAL nervous system
*VIRAL tropism
*RNA virus infections
*VIRAL physiology
*HAMSTERS
*RODENTS
*RESEARCH
*ANIMAL experimentation
*RESEARCH methodology
*RESPIRATORY infections
*MEDICAL cooperation
*EVALUATION research
*COMPARATIVE studies
*FLUORESCENCE in situ hybridization
*RESEARCH funding
*PARAMYXOVIRUSES
CENTRAL nervous system infections
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Language
ISSN
0022-1899
Abstract
Using a recombinant Nipah virus expressing a fluorescent protein (ZsG), we visualized virus tropism in the Syrian hamster model. We found that anatomical localization of fluorescence correlated to clinical signs; signal was primarily visualized in the respiratory tract in animals with acute-onset terminal disease, whereas central nervous system localization was seen in animals that succumbed with delayed disease onset. While polymerase chain reaction (PCR) detection corresponded well to ZsG signal, virus was only isolated from some lung, brain, liver, and kidney samples that were ZsG and/or PCR positive, and only from animals euthanized on or before 15 days post infection. [ABSTRACT FROM AUTHOR]