학술논문
Rhizopus microsporus Infections Associated with Surgical Procedures, Argentina, 2006-2014.
Document Type
Journal Article
Author
Bowers, Jolene R.; Monroy-Nieto, Juan; Gade, Lalitha; Travis, Jason; Refojo, Nicolás; Abrantes, Ruben; Santander, Jorge; French, Chris; Dignani, María Cecilia; Hevia, Alejandra Ines; Roe, Chandler C.; Lemmer, Darrin; Lockhart, Shawn R.; Chiller, Tom; Litvintseva, Anastasia P.; Clara, Liliana; Engelthaler, David M.
Source
Subject
*RESEARCH
*RESEARCH methodology
*FUNGI
*MEDICAL cooperation
*EVALUATION research
*COMPARATIVE studies
*MYCOSES
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Language
ISSN
1080-6040
Abstract
Rhizopus spp. fungi are ubiquitous in the environment and a rare but substantial cause of infection in immunosuppressed persons and surgery patients. During 2005-2017, an abnormally high number of Rhizopus infections in surgery patients, with no apparent epidemiologic links, were reported in Argentina. To determine the likelihood of a common source of the cluster, we performed whole-genome sequencing on samples collected during 2006-2014. Most isolates were separated by >60 single-nucleotide polymorphisms, and we found no evidence for recombination or nonneutral mutation accumulation; these findings do not support common source or patient-to-patient transmission. Assembled genomes of most isolates were ≈25 Mbp, and multiple isolates had substantially larger assembled genomes (43-51 Mbp), indicative of infections with strain types that underwent genome expansion. Whole-genome sequencing has become an essential tool for studying epidemiology of fungal infections. Less discriminatory techniques may miss true relationships, possibly resulting in inappropriate attribution of point source. [ABSTRACT FROM AUTHOR]