부산대학교 도서관

Abstract Natural and synthetic progestins in receiving streams can disrupt the normal endocrine systems of fish. Norethindrone (NET) is a widely used synthetic progestin that often appears in wastewater effluents. For this research, adult female western mosquitofish (Gambusia affinis) were exposed to NET at three concentrations. The effects of NET on the following biological factors were evaluated: the histology of the ovaries and livers, the anal fin morphology, and transcription of genes related to steroidogenesis signaling pathways in the livers. After 42 d exposure to NET at 33.0 ng L−1 and 347.5 ng L−1, rapid masculinization, an increase in the number of atretic and postovulatory follicles in the ovary, enhanced vascularization, degenerated hepatocytes and irregular nuclei in the livers were observed. Exposure to NET did not affect the expression of the androgenic and estrogenic receptor genes and Cyp19a except for a significant up-regulation of Erα. However, the expression of Vtg A, Vtg B, and Vtg C were markedly inhibited in the females exposed to three concentrations of NET. Compared to the control female, exposure to NET at 33.0 ng L−1 and 347.5 ng L−1 caused a 4.4- and 5.8-fold increase in the expression of Hsd17β3 in the livers, respectively. The results demonstrate that NET can cause rapid masculinization of female G. affinis , hepatopathological alterations and inhibited expressions of Vtg A , Vtg B , and Vtg C. The results imply that G. affinis populations might be threatened in NET-contaminated environment. Highlights • Norethindrone (NET) exposure caused rapid masculinization in female G. affinis. • NET exposure caused histopathological alterations in the ovaries and livers. • NET exposure altered the transcriptional expression of Hsd17β3 and vtg A , Vtg B , and Vtg C. [ABSTRACT FROM AUTHOR]