학술논문
RNA sequencing and differential expression reveals the effects of serial oestrus synchronisation on ovarian genes in dairy goats.
Document Type
Article
Author
Source
Subject
*GOATS
*RNA sequencing
*PROGESTATIONAL hormones
*GENE expression
*DNA damage
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Language
ISSN
1031-3613
Abstract
A total of 24 female Xinong Saanen dairy goats were used to examine differentially expressed genes (DEGs) in the ovaries of goats treated once or three times for oestrus synchronisation (ES). The goats were randomly divided into two groups: one group received three ES treatments at fortnightly intervals (repeated or triple ES group), whereas the other was only treated once on the same day as the third ES treatment for the triple group (control group) during the breeding season. Ovaries of three goats in oestrus from each group were collected for morphological examination and transcriptome sequencing, while the rest of the goats were artificially inseminated twice. Litter size and fecundity rate tended (P = 0.06) to be lower in the triple ES group. A total of 319 DEGs were identified, including carbohydrate sulphotransferase 8 (CHST8), corticosteroid-binding globulin (CBG), oestradiol 17-β-dehydrogenase 1 (DHB1), oestrogen receptor 1 (ESR1), progestin and adipoQ receptor family member 4 (PAQR4), PAQR9 , prostacyclin synthase (PTGIS), contactin-associated protein (CNTNAP4), matrix metalloproteinase-2 (MMP - 2), regulator of G-protein signalling 9-2 (RGS9 - 2) and sperm surface protein Sp17 (Sp17); these were the most promising novel candidate genes for reproductive performances in goats. Our study indicates that triple ES could cause DNA damage and alter gene expression in goat ovaries, potentially affecting ovary function, neural regulation and hormone secretion. The effects of multiple oestrus synchronisation (ES) treatments on ovarian gene expression of goats remained unknown. RNA sequencing of ovaries from goats subjected to triple ES treatments identified 319 differentially expressed genes including 11 novel genes related to reproductive performance. The results indicated that multiple ES treatments changed gene expression, potentially affected ovarian function, neural regulation and hormone secretion. [ABSTRACT FROM AUTHOR]