학술논문
Conjugated linoleic acid prevents damage caused by intestinal mucositis induced by 5-fluorouracil in an experimental model.
Document Type
Article
Author
de Barros, Patrícia Aparecida Vieira; Rabelo Andrade, Maria Emília; de Vasconcelos Generoso, Simone; Mendes Miranda, Sued Eustáquio; dos Reis, Diego Carlos; Lacerda Leocádio, Paola Caroline; de Sales e Souza, Éricka Lorenna; dos Santos Martins, Flaviano; da Gama, Marco Antonio Sundfeld; Cassali, Geovanni Dantas; Alvarez Leite, Jacqueline Isaura; Antunes Fernandes, Simone Odília; Cardoso, Valbert Nascimento
Source
Subject
*MUCOSITIS
*INTESTINAL disease treatment
*FLUOROURACIL
*LINOLEIC acid
*ANIMAL models in research
*INTESTINAL mucosa injuries
*BIOMARKERS
*THERAPEUTICS
PHYSIOLOGICAL effects of linoleic acid
*
*
*
*
*
*
*
Language
ISSN
0753-3322
Abstract
Background Studies have showed the protective effects of conjugated linoleic acid (CLA) on intestinal epithelium, modulating host immune and inflammatory responses on intestinal diseases. Objective To evaluate the preventive effects of CLA on the intestinal mucositis induced by 5-FU in a murine model. Methods Sixty-four BALB/c mice were randomly divided into four groups: Control (CTL), fed a standard chow diet; CLAs, fed a diet supplemented with CLA; Mucositis (5-FU), fed a standard chow diet and underwent mucositis induction and CLAs 5-FU, fed a diet supplemented with CLA and underwent mucositis induction. Mucositis was induced by intraperitoneal injection of 300 mg/kg 5-FU. After 72 h, the animals were euthanized and intestinal permeability, bacterial translocation, inflammatory mediators, and intestinal histology were evaluated. Results Mice in the CLAs 5-FU group showed reduced weight loss compared to those in the 5-FU group (p < 0.005). Furthermore, the results also showed that the treatment with CLA reduced intestinal permeability, bacterial translocation, and biomarkers of inflammatory response besides minor damage to ZO-1 and occludin with maintenance of the integrity of the intestinal epithelium and a favorable balance between the inflammatory and regulatory cytokines. Conclusion This study suggests that CLA reduced the adverse effects from 5-FU administration on the intestinal mucosa. [ABSTRACT FROM AUTHOR]