학술논문
Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples.
Document Type
Article
Author
Source
Subject
*DNA methylation
*SARCOIDOSIS
*HORSE diseases
*SKIN tumors
*CARCINOGENESIS
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Language
ISSN
1476-5810
Abstract
Objective: In this study, for the first time we report the genome-wide DNA methylation profile of skin tumour in horses and describe differentially methylated genomic regions (DMRs) with respect to healthy skin. Materials & Methods: The comparative analysis of DNA methylation patterns detected using Reduced Representation Bisulfite Sequencing (RRBS) technique, allowed identification of 136 regions showing differential methylation between sarcoid and normal skin tissue. Results: Most of the identified DMRs were short fragments, less than 1 kb in size, located in the intergenic regions. Among identified DMRs there were also regions located within genes directly or indirectly related with oncogenesis. We additionally validated 9 CpG sites showing hypomethylation and 9 CpG sites that were hypermethylated in lesional sample, confirming the identified changes in the DNA methylation. Conclusion: Knowledge on the changes taking place in the process of DNA methylation may provide a basis for the development of new alternative diagnostic or therapeutic approaches to equine sarcoids. [ABSTRACT FROM AUTHOR]