학술논문
Adaptation of tick-borne encephalitis virus from human brain to different cell cultures induces multiple genomic substitutions.
Document Type
Report
Author
Source
Subject
*TICK-borne encephalitis viruses
*BRAIN
*CELL culture
*SWINE embryos
*VIRAL genomes
*AMINO acid sequence
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Language
ISSN
0304-8608
Abstract
The C11-13 strain from the Siberian subtype of tick-borne encephalitis virus (TBEV) was isolated from human brain using pig embryo kidney (PEK), 293, and Neuro-2a cells. Analysis of the complete viral genome of the C11-13 variants during six passages in these cells revealed that the cell-adapted C11-13 variants had multiple amino acid substitutions as compared to TBEV from human brain. Seven out of eight amino acids substitutions in the high-replicating C11-13(PEK) variant mapped to non-structural proteins; 13 out of 14 substitutions in the well-replicating C11-13(293) variant, and all four substitutions in the low-replicating C11-13(Neuro-2a) variant were also localized in non-structural proteins, predominantly in the NS2a (2), NS3 (6) and NS5 (3) proteins. The substitutions NS2a (Asn → Asp), NS2a(Leu → Val) in the N-terminus of NS2a and NS3(His → Gln) in the helicase domain of NS3 were found in all selected variants. We postulate that multiple substitutions in the NS2a, NS3 and NS5 genes play a key role in adaptation of TBEV to different cells. [ABSTRACT FROM AUTHOR]