학술논문
Optimized incubation regime for nitric oxide measurements in murine macrophages using the Griess assay.
Document Type
Article
Author
Source
Subject
*NITRIC oxide
*MACROPHAGES
*ANTI-inflammatory agents
*DRUG use testing
*DRUG efficacy
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Language
ISSN
0022-1759
Abstract
The Griess assay is used to measure nitric oxide concentrations in liquid solutions after reaction into nitrite. The assay is challenging when applied to cell culture supernatants. During optimization, we focused on the anti-inflammatory potential of test compounds in murine RAW264.7 macrophages. This led to (i) the required inductivity of cells by lipopolysaccharide (LPS) and allowed (ii) the characterization of putative anti-inflammatory test compounds with high sensitivity. The modifications reported here prominently improved resolution and efficiency of the widely used Griess assay and are of broad interest for studies on the pharmacological modulation of macrophages activation. [ABSTRACT FROM AUTHOR]