부산대학교 도서관

This study was designed to investigate the prevalence of metallo-β-lactamase ( MBL) in Pseudomonas aeruginosa isolates collected from Suez Canal University Hospital in Ismailia, Egypt. Antibiotic susceptibility testing and phenotypic and genotypic screening for MBLs were performed on 147 isolates of P. aeruginosa. MICs were determined by agar dilution method for carbapenem that was ≥2 μg/mL for meropenem. MBL genes were detected by multiplex and monoplex PCR for P. aeruginosa-harbored plasmids. Mutation profile of sequenced MBL genes was screened using online software Clustal Omega. Out of 147 P. aeruginosa, 39 (26.5%) were carbapenem-resistant isolates and 25 (64%) were confirmed to be positive for MBLs. The susceptibility rate of P. aeruginosa toward polymyxin B and norfloxacin was 99% and 88%, respectively. Identification of collected isolates by API analysis and constructed phylogenetic tree of 16S r RNA showed that the isolates were related to P. aeruginosa species. The frequency of bla GIM-1, bla SIM-1, and bla SPM-1 was 52%, 48%, and 24%, respectively. Bla VIM and bla IMP-like genes were 20% and 4% and the sequences confirm the isolate to be bla VIM-1, bla VIM-2, bla VIM-4, and bla IMP-1. Three mutations were identified in bla VIM-4 gene. Our study emphasizes the high occurrence of multidrug-resistant P. aeruginosa-producing MBL enzymes. [ABSTRACT FROM AUTHOR]