부산대학교 도서관

Molecular epidemiology of Mycoplasma gallisepticum in commercial and backyard poultry was conducted in different parts of Pakistan and Azad Jammu and Kashmir. Molecular identification of the isolates was made with the application of polymerase chain reaction (PCR). A total of 142 isolates of M. gallisepticum from 573 flocks were identified. The isolates were further characterized on the basis of SDS-PAGE. The cumulative prevalence of M. gallisepticum noted in broilers, broiler breeders, commercial layers and layer breeders including backyard/rural poultry was 14.62, 29.33, 23.16 and 42.85%, respectively indicating significant prevalence in all type of birds with Chi-square value of X²=7.81. Significant seasonal variation (X²=3.84) indicated the higher prevalence 56.34% in winter as compared to 43.66% in summer. The findings of SDS-PAGE indicated that 71.43% of the tested isolates proved pathogenic with the presence of 74 kDa adhesive protein bands representing M. gallisepticum F strain. The remaining isolates represented less virulent or variant stains. The high prevalence of M. gallisepticum in respiratory infection of poultry in Pakistan demands for framing national policy through surveillance and control of the disease. [ABSTRACT FROM AUTHOR]