학술논문
Significance of Zone 2 Peak on Capillary Electrophoresis (ce) for the Detection of Haemoglobin Constant Spring (Hb CS) among General Population in Kelantan.
Document Type
Academic Journal
Author
Source
Subject
Language
English
ISSN
1341-2051
Abstract
Introduction: Haemoglobin Constant Spring (Hb CS) is a point mutational defect of α thalassaemia at the termination codon and leads to α chain extension and reduced mRNA stability. Hb CS is unstable haemoglobin and is low level in peripheral blood for detection. However, the capillary electrophoresis (CE) is able to separate and quantitate Hb CS. The aim of the present study was to determine the significance presence of Zone 2 peak on capillary electrophoresis (CE) in diagnosing Hb CS among general population in Kelantan. Materials and Methods: One hundred and thirty-seven patients' sample were screened by CE and proceeded with molecular analysis. In the CE electrophoregram, Hb CS and Hb Paks was detected as having a peak at Zone 2. Results: 118 samples out of 137 samples showed a positive peak in Zone 2 of CE and were also confirmed for termination codon mutation for Hb CS by molecular analysis. 19 samples showed negative results for termination codon mutation for Hb CS and termination codon mutation for Hb Paks Conclusions: Hb CS quantification can be predicted by CE method based on peak at Zone 2 if the molecular technology is not available.