학술논문
Implementing best practices on data generation and reporting of Mycobacterium tuberculosis in vitro assays within the ERA4TB consortium
Document Type
Author
Van Wijk, Rob C, 1991; Lucia, Ainhoa; Sudhakar, Pavan Kumar; Sonnenkalb, Lindsay; Gaudin, Cyril; Hoffmann, Eik; Dremierre, Berenice; Angelica Aguilar-Ayala, Diana; Dal Molin, Michael; Rybniker, Jan; de Giorgi, Stefano; Cioetto-Mazzabo, Laura; Segafreddo, Greta; Manganelli, Riccardo; Degiacomi, Giulia; Recchia, Deborah; Pasca, Maria Rosalia; Simonsson, Ulrika S. H.; Ramon-Garcia, Santiago
Source
ISCIENCE. 26(4)
Subject
Language
English
ISSN
2589-0042
Abstract
Tuberculosis (TB) is the historical leading cause of death by a single infectious agent. The European Regimen Accelerator for Tuberculosis (ERA4TB) is a public-private partnership of 30+ institutions with the objective to progress new anti-TB regimens into the clinic. Thus, robust and replicable results across independent laboratories are essential for reliable interpretation of treatment efficacy. A standardization workgroup unified in vitro protocols and data reporting templates. Time-kill assays provide essential input data for pharmacometric model-informed translation of single agents and regimens activity from in vitro to in vivo and the clinic. Five conditions were assessed by time-kill assays in six independent laboratories using four bacterial plating methods. Baseline bacterial burden varied between laboratories but variability was limited in net drug effect, confirming 2.5 mu L equally robust as 100 mu L plating. This exercise establishes the foundations of collaborative data generation, reporting, and integration within the overarching Antimicrobial Resistance Accelerator program.