학술논문

The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data
Document Type
Source
Glycobiology. 27(4):280-284
Subject
Cell and Molecular Biology
Cell- och molekylärbiologi
glycan microarrays
glycans
glycobiology
glycomics
MIRAGE
shotgun glycomics
mass-spectrometry
binding-proteins
experiment
miame
array data
receptors
oligosaccharides
neoglycolipids
specificities
biomarkers
B 440
S-40530 Gothenburg
Sweden.
[Khoo
Kay-Hooi] Acad Sinica
Inst Biol Chem
Acad Rd Sec 2
Taipei 115
Taiwan.
[Kolarich
Daniel] Max Planck Inst Colloids & Interfaces
Dept Biomol Syst
D-14424 Potsdam
Germany.
[Novotny
Milos V.] Indiana Univ
Dept Chem
800 E Kirkwood Ave
Bloomington
IN 47405 USA.
[Ranzinger
Rene
Tiemeyer
Michael
Wells
Lance
York
William S.] Univ Georgia
Complex
[Rapp
Erdmann] Max Planck Inst Dynam Complex Tech Syst
Bioproc Engn
D-39106 Magdeburg
[Rudd
Pauline M.] NIBRT Natl Inst Bioproc Res & Training
NIBRT GlycoSci Grp
Fosters Ave
Blackrock
[Struwe
Weston B.] Univ Oxford
Glycobiol Inst
Dept Biochem
Oxford OX1 3QU
England.
[Kettner
Carsten] Beilstein Inst
Trakehner Str 7-9
D-60487 Frankfurt
[Smith
David F.] Emory Univ
Sch Med
Emory Comprehens Glyc Core
Atlanta
GA 30322 USA.
ng pw
biochemical and biophysical research communications
v132
p474
Language
English
ISSN
0959-6658
Abstract
MIRAGE (Minimum Information Required for A Glycomics Experiment) is an initiative that was created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to produce guidelines for reporting results from the diverse types of experiments and analyses used in structural and functional studies of glycans in the scientific literature. As a sequel to the guidelines for sample preparation (Struwe et al. 2016, Glycobiology, 26: 907-910) and mass spectrometry data (Kolarich et al. 2013, Mol. Cell Proteomics, 12: 991-995), here we present the first version of guidelines intended to improve the standards for reporting data from glycan microarray analyses. For each of eight areas in the workflow of a glycan microarray experiment, we provide guidelines for the minimal information that should be provided in reporting results. We hope that the MIRAGE glycan microarray guidelines proposed here will gain broad acceptance by the community, and will facilitate interpretation and reproducibility of the glycan microarray results with implications in comparison of data from different laboratories and eventual deposition of glycan microarray data in international databases.