학술논문
Ultrafast structural changes within a photosynthetic reaction centre.
Document Type
Author
Dods, Robert, 1989; Båth, Petra, 1988; Morozov, Dmitry; Ahlberg Gagnér, Viktor; Arnlund, David; Luk, Hoi Ling; Kübel, Joachim, 1988; Maj, Michal; Vallejos, Adams, 1987; Wickstrand, Cecilia; Bosman, Robert, 1991; Beyerlein, Kenneth R; Nelson, Garrett; Liang, Mengning; Milathianaki, Despina; Robinson, Joseph; Harimoorthy, Rajiv; Berntsen, Peter, 1974; Malmerberg, Erik; Johansson, Linda C, 1983; Andersson, Rebecka, 1988; Carbajo, Sergio; Claesson, Elin, 1989; Conrad, Chelsie E; Dahl, Peter, 1965; Hammarin, Greger, 1981; Hunter, Mark S; Li, Chufeng; Lisova, Stella; Royant, Antoine; Safari, Cecilia, 1989; Sharma, Amit; Williams, Garth J; Yefanov, Oleksandr; Westenhoff, Sebastian, 1978; Davidsson, Jan; DePonte, Daniel P; Boutet, Sébastien; Barty, Anton; Katona, Gergely, 1975; Groenhof, Gerrit; Brändén, Gisela, 1975; Neutze, Richard, 1969
Source
Nature. 589(7841):310-314
Subject
Language
English
ISSN
1476-4687
Abstract
Photosynthetic reaction centres harvest the energy content of sunlight by transporting electrons across an energy-transducing biological membrane. Here we use time-resolved serial femtosecond crystallography1 using an X-ray free-electron laser2 to observe light-induced structural changes in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds. Structural perturbations first occur at the special pair of chlorophyll molecules of the photosynthetic reaction centre that are photo-oxidized by light. Electron transfer to the menaquinone acceptor on the opposite side of the membrane induces a movement of this cofactor together with lower amplitude protein rearrangements. These observations reveal how proteins use conformational dynamics to stabilize the charge-separation steps of electron-transfer reactions.