학술논문
Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids
Document Type
Author
Bojmar, Linda, 1983; Kim, Han Sang; Tobias, Gabriel C; Pelissier Vatter, Fanny A; Lucotti, Serena; Gyan, Kofi Ennu; Kenific, Candia M.; Wan, Zurong; Kim, Kyung-A; Kim, DooA; Hernandez, Jonathan; Pascual, Virginia; Heaton, Todd E; La Quaglia, Michael P; Kelsen, David; Trippett, Tanya M; Jones, David R; Jarnagin, William R.; Matei, Irina R.; Zhang, Haiying; Hoshino, Ayuko; Lyden, David
Source
STAR protocols. 2(1)
Subject
Language
English
ISSN
2666-1667
Abstract
We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020).