학술논문
Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source
Document Type
Author
Leonarski, Filip; Nan, Jie; Matej, Zdenek; Bertrand, Quentin; Furrer, Antonia; Gorgisyan, Ishkhan; Bjelčić, Monika; Kepa, Michal; Glover, Hannah; Hinger, Viktoria; Eriksson, Thomas; Cehovin, Aleksander; Eguiraun, Mikel; Gasparotto, Piero; Mozzanica, Aldo; Weinert, Tobias; Gonzalez, Ana; Standfuss, Jörg; Wang, Meitian; Ursby, Thomas; Dworkowski, Florian
Source
IUCrJ eSSENCE: The e-Science Collaboration. 10(Pt 6):729-737
Subject
Language
English
ISSN
2052-2525
Abstract
Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most thirdgeneration synchrotron-based macromolecular crystallography beamlines do not offer the necessary infrastructure yet. Here, a new setup is demonstrated, based on the JUNGFRAU detector and Jungfraujoch data-acquisition system, that enables collection of kilohertz serial crystallography data at fourthgeneration synchrotrons. More importantly, it is shown that this setup is capable of collecting multiple-time-point time-resolved protein dynamics at kilohertz rates, allowing the probing of microsecond to second dynamics at synchrotrons in a fraction of the time needed previously. A high-quality complete X-ray dataset was obtained within 1 min from lysozyme microcrystals, and the dynamics of the light-driven sodium-pump membrane protein KR2 with a time resolution of 1 ms could be demonstrated. To make the setup more accessible for researchers, downstream data handling and analysis will be automated to allow on-the-fly spot finding and indexing, as well as data processing.