학술논문
Disturbances in microbial skin recolonization and cutaneous immune response following allogeneic stem cell transfer
Document Type
Original Paper
Author
Bayer, Nadine; Hausman, Bela; Pandey, Ram Vinay; Deckert, Florian; Gail, Laura-Marie; Strobl, Johanna; Pjevac, Petra; Krall, Christoph; Unterluggauer, Luisa; Redl, Anna; Bachmayr, Victoria; Kleissl, Lisa; Nehr, Marion; Kirkegaard, Rasmus; Makristathis, Athanasios; Watzenboeck, Martin L.; Nica, Robert; Staud, Clement; Hammerl, Lukas; Wohlfarth, Philipp; Ecker, Rupert C.; Knapp, Sylvia; Rabitsch, Werner; Berry, David; Stary, Georg
Source
Leukemia. 36(11):2705-2714
Subject
Language
English
ISSN
0887-6924
1476-5551
1476-5551
Abstract
The composition of the gut microbiome influences the clinical course after allogeneic hematopoietic stem cell transplantation (HSCT), but little is known about the relevance of skin microorganisms. In a single-center, observational study, we recruited a cohort of 50 patients before undergoing conditioning treatment and took both stool and skin samples up to one year after HSCT. We could confirm intestinal dysbiosis following HSCT and report that the skin microbiome is likewise perturbed in HSCT-recipients. Overall bacterial colonization of the skin was decreased after conditioning. Particularly patients that developed acute skin graft-versus-host disease (aGVHD) presented with an overabundance of Staphylococcus spp. In addition, a loss in alpha diversity was indicative of aGVHD development already before disease onset and correlated with disease severity. Further, co-localization of CD45+ leukocytes and staphylococci was observed in the skin of aGVHD patients even before disease development and paralleled with upregulated genes required for antigen-presentation in mononuclear phagocytes. Overall, our data reveal disturbances of the skin microbiome as well as cutaneous immune response in HSCT recipients with changes associated with cutaneous aGVHD.