학술논문
SARS-CoV-2 and other respiratory pathogens are detected in continuous air samples from congregate settings
Document Type
Original Paper
Author
Ramuta, Mitchell D.; Newman, Christina M.; Brakefield, Savannah F.; Stauss, Miranda R.; Wiseman, Roger W.; Kita-Yarbro, Amanda; O’Connor, Eli J.; Dahal, Neeti; Lim, Ailam; Poulsen, Keith P.; Safdar, Nasia; Marx, John A.; Accola, Molly A.; Rehrauer, William M.; Zimmer, Julia A.; Khubbar, Manjeet; Beversdorf, Lucas J.; Boehm, Emma C.; Castañeda, David; Rushford, Clayton; Gregory, Devon A.; Yao, Joseph D.; Bhattacharyya, Sanjib; Johnson, Marc C.; Aliota, Matthew T.; Friedrich, Thomas C.; O’Connor, David H.; O’Connor, Shelby L.
Source
Nature Communications. 13(1)
Subject
Language
English
ISSN
2041-1723
Abstract
Two years after the emergence of SARS-CoV-2, there is still a need for better ways to assess the risk of transmission in congregate spaces. We deployed active air samplers to monitor the presence of SARS-CoV-2 in real-world settings across communities in the Upper Midwestern states of Wisconsin and Minnesota. Over 29 weeks, we collected 527 air samples from 15 congregate settings. We detected 106 samples that were positive for SARS-CoV-2 viral RNA, demonstrating that SARS-CoV-2 can be detected in continuous air samples collected from a variety of real-world settings. We expanded the utility of air surveillance to test for 40 other respiratory pathogens. Surveillance data revealed differences in timing and location of SARS-CoV-2 and influenza A virus detection. In addition, we obtained SARS-CoV-2 genome sequences from air samples to identify variant lineages. Collectively, this shows air sampling is a scalable, high throughput surveillance tool that could be used in conjunction with other methods for detecting respiratory pathogens in congregate settings.
Air surveillance offers a potential means of monitoring airborne pathogens without the need for individual sampling. Here, the authors perform continuous air sampling in 15 community settings in the US for 29 weeks and demonstrate its feasibility for routine detection of SARS-CoV-2 and other respiratory pathogens.
Air surveillance offers a potential means of monitoring airborne pathogens without the need for individual sampling. Here, the authors perform continuous air sampling in 15 community settings in the US for 29 weeks and demonstrate its feasibility for routine detection of SARS-CoV-2 and other respiratory pathogens.