학술논문
Viral isolation allows characterization of early samples of SARS-CoV-2 lineage B1.1.33 with unique mutations (S: H655Y and T63N) circulating in Southern Brazil in 2020
Document Type
Original Paper
Author
Gularte, Juliana Schons; da Silva, Mariana Soares; Filippi, Micheli; Demoliner, Meriane; Schallenberger, Karoline; Hansen, Alana Witt; de Abreu Góes Pereira, Vyctoria Malayhka; Heldt, Fágner Henrique; Girardi, Viviane; Weber, Matheus Nunes; de Almeida, Paula Rodrigues; Abbadi, Bruno Lopes; Dornelles, Maiele; Bizarro, Cristiano Valim; Machado, Pablo; Basso, Luiz Augusto; Dellagostin, Odir Antonio; Fleck, Juliane Deise; Spilki, Fernando Rosado
Source
Brazilian Journal of Microbiology. 53(3):1313-1319
Subject
Language
English
ISSN
1517-8382
1678-4405
1678-4405
Abstract
Different approaches are in use to improve our knowledge about the causative agent of coronavirus disease (COVID-19). Cell culture-based methods are the better way to perform viral isolation, evaluate viral infectivity, and amplify the virus. Furthermore, next-generation sequencing (NGS) have been essential to analyze a complete genome and to describe new viral species and lineages that have arisen over time. Four naso-oropharyngeal swab samples, collected from April to July of 2020, were isolated and sequenced aiming to produce viral stocks and analyze the mutational profile of the found lineage. B.1.1.33 was the lineage detected in all sequences. Although the samples belong to the same lineage, it was possible to evaluate different mutations found including some that were first described in these sequences, like the S:H655Y and T63N. The results described here can help to elicit how the pandemic started to spread and how it has been evolving in south Brazil.