학술논문
High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler
Document Type
Original Paper
Author
Source
Nature Biotechnology: The Science and Business of Biotechnology. 42(4):587-590
Subject
Language
English
ISSN
1087-0156
1546-1696
1546-1696
Abstract
We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.
Lipid nanoparticles, viruses, exosomes and liposomes are characterized by analysis of fluorescence fluctuations.
Lipid nanoparticles, viruses, exosomes and liposomes are characterized by analysis of fluorescence fluctuations.