학술논문
Detection of Streptococcus pyogenes M1UK in Australia and characterization of the mutation driving enhanced expression of superantigen SpeA
Document Type
Original Paper
Author
Davies, Mark R.; Keller, Nadia; Brouwer, Stephan; Jespersen, Magnus G.; Cork, Amanda J.; Hayes, Andrew J.; Pitt, Miranda E.; De Oliveira, David M. P.; Harbison-Price, Nichaela; Bertolla, Olivia M.; Mediati, Daniel G.; Curren, Bodie F.; Taiaroa, George; Lacey, Jake A.; Smith, Helen V.; Fang, Ning-Xia; Coin, Lachlan J. M.; Stevens, Kerrie; Tong, Steven Y. C.; Sanderson-Smith, Martina; Tree, Jai J.; Irwin, Adam D.; Grimwood, Keith; Howden, Benjamin P.; Jennison, Amy V.; Walker, Mark J.
Source
Nature Communications. 14(1)
Subject
Language
English
ISSN
2041-1723
Abstract
A new variant of Streptococcus pyogenes serotype M1 (designated ‘M1UK ’) has been reported in the United Kingdom, linked with seasonal scarlet fever surges, marked increase in invasive infections, and exhibiting enhanced expression of the superantigen SpeA. The progenitor S. pyogenes ‘M1global ’ and M1UK clones can be differentiated by 27 SNPs and 4 indels, yet the mechanism for speA upregulation is unknown. Here we investigate the previously unappreciated expansion of M1UK in Australia, now isolated from the majority of serious infections caused by serotype M1 S. pyogenes. M1UK sub-lineages circulating in Australia also contain a novel toxin repertoire associated with epidemic scarlet fever causing S. pyogenes in Asia. A single SNP in the 5’ transcriptional leader sequence of the transfer-messenger RNA gene ssrA drives enhanced SpeA superantigen expression as a result of ssrA terminator read-through in the M1UK lineage. This represents a previously unappreciated mechanism of toxin expression and urges enhanced international surveillance.
A variant of group A Streptococcus serotype M1 (UK) has been increasingly reported and can be differentiated from the global variant by its overexpression of the superantigen SpeA. Here, Davies et al probe the mechanism behind enhanced SpeA expression and demonstrate that a SNP in the 5’ leader sequence of ssrA is responsible for this virulence phenotype.
A variant of group A Streptococcus serotype M1 (UK) has been increasingly reported and can be differentiated from the global variant by its overexpression of the superantigen SpeA. Here, Davies et al probe the mechanism behind enhanced SpeA expression and demonstrate that a SNP in the 5’ leader sequence of ssrA is responsible for this virulence phenotype.