부산대학교 도서관

It is shown that two new methods can be used to quantitate the intracellular accretion and interaction with DNA of any anthracycline antibiotics (AA) during short-term incubation of vital cells with preparation. The intracellular accretion of doxorubicin encapsulated in polymeric nanospheres (NS-DR) is significantly less than that of free DR. NSDR and NS do not thereby affect the intracellular accretion of DR. Thus, qualitative and quantitative differences in the activity of free AA and their complexes with polymers may be related to the differences in their intracellular accretion and to the possibility of realizing biological effects of the complexes without penetrating inside the cell.