학술논문
A Roadmap for the Human Gut Cell Atlas
Document Type
Review Paper
Author
Zilbauer, Matthias; James, Kylie R.; Kaur, Mandeep; Pott, Sebastian; Li, Zhixin; Burger, Albert; Thiagarajah, Jay R.; Burclaff, Joseph; Jahnsen, Frode L.; Perrone, Francesca; Ross, Alexander D.; Matteoli, Gianluca; Stakenborg, Nathalie; Sujino, Tomohisa; Moor, Andreas; Bartolome-Casado, Raquel; Bækkevold, Espen S.; Zhou, Ran; Xie, Bingqing; Lau, Ken S.; Din, Shahida; Magness, Scott T.; Yao, Qiuming; Beyaz, Semir; Arends, Mark; Denadai-Souza, Alexandre; Coburn, Lori A.; Gaublomme, Jellert T.; Baldock, Richard; Papatheodorou, Irene; Ordovas-Montanes, Jose; Boeckxstaens, Guy; Hupalowska, Anna; Teichmann, Sarah A.; Regev, Aviv; Xavier, Ramnik J.; Simmons, Alison; Snyder, Michael P.; Wilson, Keith T.
Source
Nature Reviews Gastroenterology & Hepatology. 20(9):597-614
Subject
Language
English
ISSN
1759-5045
1759-5053
1759-5053
Abstract
The number of studies investigating the human gastrointestinal tract using various single-cell profiling methods has increased substantially in the past few years. Although this increase provides a unique opportunity for the generation of the first comprehensive Human Gut Cell Atlas (HGCA), there remains a range of major challenges ahead. Above all, the ultimate success will largely depend on a structured and coordinated approach that aligns global efforts undertaken by a large number of research groups. In this Roadmap, we discuss a comprehensive forward-thinking direction for the generation of the HGCA on behalf of the Gut Biological Network of the Human Cell Atlas. Based on the consensus opinion of experts from across the globe, we outline the main requirements for the first complete HGCA by summarizing existing data sets and highlighting anatomical regions and/or tissues with limited coverage. We provide recommendations for future studies and discuss key methodologies and the importance of integrating the healthy gut atlas with related diseases and gut organoids. Importantly, we critically overview the computational tools available and provide recommendations to overcome key challenges.
Single-cell profiling studies of the human gastrointestinal tract are increasing, offering an excellent opportunity to generate the first Human Gut Cell Atlas. This Roadmap presents a structured direction towards this goal and provides a detailed overview of the major challenges.
Key points: The number of studies applying single-cell sequencing methods to human intestinal tissue has been rapidly increasing, providing a unique opportunity to generate a complete map of the human intestine.Generation of the Human Gut Cell Atlas (HGCA) requires the coordinated efforts of groups across the globe and the integration of various data sets followed by their computational analyses.This article provides a roadmap for the generation of the HGCA based on the expertise and recommendations of the Gut Biological Network of the Human Cell Atlas.The HGCA will provide a unique and highly valuable reference map enhancing research in intestinal health and disease.
Single-cell profiling studies of the human gastrointestinal tract are increasing, offering an excellent opportunity to generate the first Human Gut Cell Atlas. This Roadmap presents a structured direction towards this goal and provides a detailed overview of the major challenges.
Key points: The number of studies applying single-cell sequencing methods to human intestinal tissue has been rapidly increasing, providing a unique opportunity to generate a complete map of the human intestine.Generation of the Human Gut Cell Atlas (HGCA) requires the coordinated efforts of groups across the globe and the integration of various data sets followed by their computational analyses.This article provides a roadmap for the generation of the HGCA based on the expertise and recommendations of the Gut Biological Network of the Human Cell Atlas.The HGCA will provide a unique and highly valuable reference map enhancing research in intestinal health and disease.