학술논문
Anticancer effects of phytol against Sarcoma (S-180) and Human Leukemic (HL-60) cancer cells
Document Type
Original Paper
Author
de Alencar, Marcus Vinícius Oliveira Barros; Islam, Muhammad Torequl; da Mata, Ana Maria Oliveira Ferreira; dos Reis, Antonielly Campinho; de Lima, Rosália Maria Torres; de Oliveira Ferreira, José Roberto; de Castro e Sousa, João Marcelo; Ferreira, Paulo Michel Pinheiro; de Carvalho Melo-Cavalcante, Ana Amélia; Rauf, Abdur; Hemeg, Hassan A.; Alsharif, Khalaf F.; Khan, Haroon
Source
Environmental Science and Pollution Research. 30(33):80996-81007
Subject
Language
English
ISSN
1614-7499
Abstract
Phytol (Pyt), a diterpenoid, possesses many important bioactivities. This study evaluates the anticancer effects of Pyt on sarcoma 180 (S-180) and human leukemia (HL-60) cell lines. For this purpose, cells were treated with Pyt (4.72, 7.08, or 14.16 μM) and a cell viability assay was performed. Additionally, the alkaline comet assay and micronucleus test with cytokinesis were also performed using doxorubicin (6 μM) and hydrogen peroxide (10 mM) as positive controls and stressors, respectively. Results revealed that Pyt significantly reduced the viability and rate of division in S-180 and HL-60 cells with IC50 values of 18.98 ± 3.79 and 1.17 ± 0.34 μM, respectively. Pyt at 14.16 μM exerted aneugenic and/or clastogenic effects in S-180 and HL-60 cells, where the number of micronuclei and other nuclear abnormalities (e.g., nucleoplasmic bridges and nuclear buds) were frequently observed. Moreover, Pyt at all concentrations induced apoptosis and showed necrosis at 14.16 μM, suggesting its anticancer effects on the tested cancer cell lines. Taken together, Pyt showed promising anticancer effects, possibly through inducing apoptosis and necrosis mechanisms, and it exerted aneugenic and/or clastogenic effects on the S-180 and HL-60 cell lines.