학술논문
Cocoa Bean Shell Wastes for the Green Synthesis of Silver Nanoparticles with Antimicrobial Activity Against Foodborne Pathogens
Document Type
Original Paper
Author
Santos, Ingrid Alves; de Barros, Hanna Elisia Araújo; de Abreu, Danilo José Machado; Sampaio, Igor Carvalho Fontes; Pereira, Elano Pinheiro; dos Reis Feliciano, Cristiane; Santos, Mariane Gonçalves; de Oliveira, Juliano Elvis; Piccoli, Roberta Hilsdorf; Irfan, Muhammad; de Barros Vilas Boas, Eduardo Valério; Franco, Marcelo
Source
Food and Bioprocess Technology: An International Journal. :1-13
Subject
Language
English
ISSN
1935-5130
1935-5149
1935-5149
Abstract
The green synthesis of silver nanoparticles offers a non-toxic, eco-friendly, economical, and efficient solution against various microorganisms. The study aimed to investigate the utilization of cocoa bean shells for the production of silver nanoparticles through green synthesis, besides its characterization and evaluation against foodborne pathogens. To this end, total phenolic compounds, individual bioactive compounds, and antioxidant activity were evaluated in the aqueous extract of the cocoa bean shell. In addition, the size and stability of the silver nanoparticles were investigated (zeta potential and dynamic light scattering), morphology (scanning electron microscopy), chemical composition (mid-infrared spectroscopy), and their effectiveness against Escherichia coli (ATCC 25922), Listeria monocytogenes (ATCC 19117), Staphylococcus aureus (ATCC 22923), Salmonella enteritidis (S64), Cronobacter sakazakii (ATCC 29004), and Klebsiella pneumoniae (ATCC 18883). The extract was rich in reducing agents and efficiently produced AgNPs with high surface area (74.51 ± 0.38 nm) and stability (− 17.97 ± 0.12 mV). The nanoparticles were also found to be effective against all tested pathogens, demonstrating the potential of agro-industrial waste to produce AgNPs for various industrial applications.Graphical Abstract: