학술논문
Differential SLC6A4 methylation: a predictive epigenetic marker of adiposity from birth to adulthood
Document Type
Original Paper
Author
Lillycrop, Karen A.; Garratt, Emma S.; Titcombe, Philip; Melton, Phillip E.; Murray, Robert J. S.; Barton, Sheila J.; Clarke-Harris, Rebecca; Costello, Paula M.; Holbrook, Joanna D.; Hopkins, James C.; Childs, Caroline E.; Paras-Chavez, Carolina; Calder, Philip C.; Mori, Trevor A.; Beilin, Lawrie; Burdge, Graham C.; Gluckman, Peter D.; Inskip, Hazel M.; Harvey, Nicholas C.; Hanson, Mark A.; Huang, Rae-Chi; Cooper, Cyrus; EpiGen Consortium; Godfrey, Keith M.
Source
International Journal of Obesity: Official journal of the International Association for the Study of Obesity. 43(5):974-988
Subject
Language
English
ISSN
0307-0565
1476-5497
1476-5497
Abstract
Background: The early life environment may influence susceptibility to obesity and metabolic disease in later life through epigenetic processes. SLC6A4 is an important mediator of serotonin bioavailability, and has a key role in energy balance. We tested the hypothesis that methylation of the SLC6A4 gene predicts adiposity across the life course.Methods: DNA methylation at 5 CpGs within the SLC6A4 gene identified from a previous methyl binding domain array was measured by pyrosequencing. We measured DNA methylation in umbilical cord (UC) from children in the Southampton Women’s Survey cohort (n = 680), in peripheral blood from adolescents in the Western Australian Pregnancy Cohort Study (n = 812), and in adipose tissue from lean and obese adults from the UK BIOCLAIMS cohort (n = 81). Real-time PCR was performed to assess whether there were corresponding alterations in gene expression in the adipose tissue.Results: Lower UC methylation of CpG5 was associated with higher total fat mass at 4 years (p = 0.031), total fat mass at 6–7 years (p = 0.0001) and % fat mass at 6–7 years (p = 0.004). Lower UC methylation of CpG5 was also associated with higher triceps skinfold thickness at birth (p = 0.013), 6 months (p = 0.038), 12 months (p = 0.062), 2 years (p = 0.0003), 3 years (p = 0.00004) and 6–7 years (p = 0.013). Higher maternal pregnancy weight gain (p = 0.046) and lower parity (p = 0.029) were both associated with lower SLC6A4 CpG5 methylation. In adolescents, lower methylation of CpG5 in peripheral blood was associated with greater concurrent measures of adiposity including BMI (p ≤ 0.001), waist circumference (p = 0.011), subcutaneous fat (p ≤ 0.001) and subscapular, abdominal and suprailiac skinfold thicknesses (p = 0.002, p = 0.008, p = 0.004, respectively). In adipose tissue, methylation of both SLC6A4 CpG5 (p = 0.019) and expression of SLC6A4 (p = 0.008) was lower in obese compared with lean adults.Conclusions: These data suggest that altered methylation of CpG loci within SLC6A4 may provide a robust marker of adiposity across the life course.