학술논문
Neutrophil Activation and Immune Thrombosis Profiles Persist in Convalescent COVID-19
Document Type
Original Paper
Author
Hocini, Hakim; Wiedemann, Aurélie; Blengio, Fabiola; Lefebvre, Cécile; Cervantes-Gonzalez, Minerva; Foucat, Emile; Tisserand, Pascaline; Surenaud, Mathieu; Coléon, Séverin; Prague, Mélanie; Guillaumat, Lydia; Krief, Corinne; Fenwick, Craig; Laouénan, Cédric; Bouadma, Lila; Ghosn, Jade; Pantaleo, Giuseppe; Thiébaut, Rodolphe; Lévy, Yves; Abel, Laurent; Abrous, Amal; Andrejak, Claire; Angoulvant, François; Bachelet, Delphine; Bartoli, Marie; Behilill, Sylvie; Beluze, Marine; Bhavsar, Krishna; Chair, Anissa; Charpentier, Charlotte; Chenard, Léo; Chirouze, Catherine; Couffin-cadiergues, Sandrine; Couffignal, Camille; Castro, Nathalie DE.; Debray, Marie-Pierre; Deplanque, Dominique; Descamps, Diane; Diallo, Alpha; Silva, Fernanda Dias DA; Dorival, Céline; Duval, Xavier; Eloy, Philippine; Enouf, Vincent; Esperou, Hélène; Esposito-farese, Marina; Etienne, Manuel; Florence, Aline-Marie; Gaymard, Alexandre; Gigante, Tristan; Gilg, Morgane; Goehringer, François; Guedj, Jérémie; Houas, Ikram; Hoffmann, Isabelle; Hulot, Jean-Sébastien; Jaafoura, Salma; Jamard, Simon; Kafif, Ouifiya; Khalil, Antoine; Lafhej, Nadhem; Laribi, Samira; Le, Minh; Hingrat, Quentin LE.; Mestre, Soizic LE.; Letrou, Sophie; Lina, Bruno; Lingas, Guillaume; Malvy, Denis; Mentré, France; Mouquet, Hugo; Neant, Nadège; Paul, Christelle; Papadopoulos, Aurélie; Petrov-sanchez, Ventzislava; Peytavin, Gilles; Piquard, Valentine; Picone, Olivier; Rosa-calatrava, Manuel; Rossignol, Bénédicte; Rossignol, Patrick; Roy, Carine; Schneider, Marion; Tardivon, Coralie; Timsit, Jean-François; Tubiana, Sarah; Werf, Sylvie VAN. DER.; Visseaux, Benoit
Source
Journal of Clinical Immunology: International Journal of Inborn Errors of Immunity and Related Diseases. :1-13
Subject
Language
English
ISSN
0271-9142
1573-2592
1573-2592
Abstract
Purpose: Following a severe COVID-19 infection, a proportion of individuals develop prolonged symptoms. We investigated the immunological dysfunction that underlies the persistence of symptoms months after the resolution of acute COVID-19.Methods: We analyzed cytokines, cell phenotypes, SARS-CoV-2 spike-specific and neutralizing antibodies, and whole blood gene expression profiles in convalescent severe COVID-19 patients 1, 3, and 6 months following hospital discharge.Results: We observed persistent abnormalities until month 6 marked by (i) high serum levels of monocyte/macrophage and endothelial activation markers, chemotaxis, and hematopoietic cytokines; (ii) a high frequency of central memory CD4+ and effector CD8+ T cells; (iii) a decrease in anti-SARS-CoV-2 spike and neutralizing antibodies; and (iv) an upregulation of genes related to platelet, neutrophil activation, erythrocytes, myeloid cell differentiation, and RUNX1 signaling. We identified a “core gene signature” associated with a history of thrombotic events, with upregulation of a set of genes involved in neutrophil activation, platelet, hematopoiesis, and blood coagulation.Conclusion: The lack of restoration of gene expression to a normal profile after up to 6 months of follow-up, even in asymptomatic patients who experienced severe COVID-19, signals the need to carefully extend their clinical follow-up and propose preventive measures.