부산대학교 도서관

Marine-derived bioactive molecules are rich sources of structurally and chemically diverse molecules for industrial and biomedical applications. In this study, Bacillus enclensis CARE-V7 was isolated from the southeastern coast of India to determine its genome sequence. Secondary metabolite gene clusters were identified using an anti-SMASH server. The bioactive compound was fractionated by gel column chromatography, and the antibacterial activity was studied by human pathogenic bacterial strains. FTIR and NMR spectroscopy were used to describe the structural characteristics of the active metabolite molecules. 3-Benzyl-6-isobutyl-2, 5-dioxo-piperazine (25.65%), and 3, 6-diisobutyl-2, 5-piperazinedione (8.29%) were both present in the ethyl acetate extract based on the GC-MS profile. The fractionated active metabolites showed greater antibacterial properties against Pseudomonas aeruginosa and Escherichia coli. In zebrafish embryos (ZFE), the teratogenicity of the active ingredient was also investigated. When the bioactive metabolites were detected at low concentrations (25 and 50 μg/ml), the teratogenicity results revealed no embryotoxicity.