부산대학교 도서관

The present study investigated the interspecific variability of organic volatiles, the antifungal and insect repellency activities of the essential oils from two subspecies of Eryngium tricuspidatum; E. tricuspidatum subsp. tricuspidatum (E.t.S.t) and E. tricuspidatum subsp. bovei (E.t.S.b) (an Algerian-Tunisian endemic taxon) growing wild in Tunisia. The phytochemical characterization was carried out by using the hybrid analytical technique: gas chromatography paired with mass spectrometry (GC/MS), combined with gas chromatography-flame ionization detection (GC/FID). As a result, fifteen and nineteen compounds representing 80.3% and 90.2% of the total volatiles were identified in subspecies bovei and tricuspidatum oils, respectively. Importantly, both analytes were distinguished by a high proportion of sesquiterpene hydrocarbons 61.1% in (E.t.S.b) and 46.4% in (E.t.S.t). β-bisabolenal (17.8%), bicyclogermacrene (11.9%) and trans-muurola-3,5-diene (11.5%) were detected as the main odorants of tricuspidatum subspecies, whereas bovei essential oil was mainly composed of bicyclogermacrene (27.1%) and trans-muurola-3,5-diene (20.9%). The antifungal potential of the tested oils was evaluated towards three fungal species: Candida albicans, C. glabrata and Trichophyton rubrum. Both essential oils manifested promising anti-candidal activity against C. albicans as their Minimum Inhibitory Concentrations values (MIC50) were less than 0.25 mg.mL− 1. In addition, the volatile oil obtained from subspecies bovei demonstrated the most potent inhibitory activity against the dermatophytic fungus T. rubrum, with MIC50 value of 0.5 mg.mL− 1. The repellency and toxicity of these oils were assayed towards T. confusum, the primary insect pest of stored cereals. Notably, the repellent effects achieved (50 and 60)% for tricuspidatum and bovei, respectively.