학술논문
Bright and stable monomeric green fluorescent protein derived from StayGold
Document Type
Original Paper
Author
Zhang, Hanbin; Lesnov, Gleb D.; Subach, Oksana M.; Zhang, Wenhao; Kuzmicheva, Tatyana P.; Vlaskina, Anna V.; Samygina, Valeriya R.; Chen, Liangyi; Ye, Xianxin; Nikolaeva, Alena Yu.; Gabdulkhakov, Azat; Papadaki, Stavrini; Qin, Wenming; Borshchevskiy, Valentin; Perfilov, Maxim M.; Gavrikov, Alexey S.; Drobizhev, Mikhail; Mishin, Alexander S.; Piatkevich, Kiryl D.; Subach, Fedor V.
Source
Nature Methods: Techniques for life scientists and chemists. 21(4):657-665
Subject
Language
English
ISSN
1548-7091
1548-7105
1548-7105
Abstract
The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging; however, its dimeric nature precludes its application as a fluorescent tag for some proteins. Here, we report the development and crystal structures of a monomeric variant of StayGold, named mBaoJin, which preserves the beneficial properties of its precursor, while serving as a tag for structural proteins and membranes. Systematic benchmarking of mBaoJin against popular green fluorescent proteins and other recently introduced monomeric and pseudomonomeric derivatives of StayGold established mBaoJin as a bright and photostable fluorescent protein, exhibiting rapid maturation and high pH/chemical stability. mBaoJin was also demonstrated for super-resolution, long-term live-cell imaging and expansion microscopy. We further showed the applicability of mBaoJin for neuronal labeling in model organisms, including Caenorhabditis elegans and mice.
mBaoJin is a monomeric derivative of the bright and photostable green fluorescent protein StayGold. mBaoJin offers favorable photophysical properties for use in diverse protein tagging and subcellular labeling applications.
mBaoJin is a monomeric derivative of the bright and photostable green fluorescent protein StayGold. mBaoJin offers favorable photophysical properties for use in diverse protein tagging and subcellular labeling applications.