학술논문
Neue Methoden der multiparametrischen Gewebediagnostik
Document Type
Review Paper
Author
Source
Die Onkologie: Organ der Deutschen Krebsgesellschaft e.V.. 29(12):1069-1077
Subject
Language
German
ISSN
2731-7226
2731-7234
2731-7234
Abstract
In recent years, various methods of multiparametric tissue diagnostics have been developed that allow a detailed molecular, cellular, and architectural characterization of the microenvironment of solid tumors. Fluorescence-based methods such as tyramide signal amplification as well as novel mass spectrometric techniques like imaging mass cytometry enable the simultaneous analysis of a large number of protein markers in a tissue section. Cyclic immunostaining and DNA barcoding approaches can theoretically even analyze unlimited targets. These methods provide fascinating insights into the complexity of tumor biology but also raise challenges regarding sampling, data analysis and clinical validation.