학술논문
The efficiency of fatty acids, N-acetyl-D-mannosamine, and N-acetylneuraminic acid for a change in the sialylation profile of recombinant darbepoetin alfa in CHO cell culture
Document Type
Original Paper
Author
Source
Applied Biochemistry and Microbiology. December 2015 51(8):827-833
Subject
Language
English
ISSN
0003-6838
1608-3024
1608-3024
Abstract
A combination of cultural medium and nourishment providing a high yield of target glycated forms of recombinant darbepoetin alfa produced by CHO cell culture was selected. The influence of different substances—precursors of glycans in glycosylation reactions—on the yield of highly sialylated forms of recombinant darbepoetin alfa was researched. It was shown that the use of 20 mM N-acetyl-D-mannosamine is the most effective for an increase in the concentration of highly sialylated forms of the protein in cultural fluid.