학술논문

Development of a microsatellite marker resource for the thin-lipped mullet, Chelon ramada (Risso, 1827)
Document Type
Brief Communication
Source
Biologia: Botany, Zoology and Cellular and Molecular Biology. :1-6
Subject
Microsatellite
High-throughput sequencing
Mugilids
Mediterranean Sea
Resource sharing
Language
English
ISSN
1336-9563
Abstract
Thin-lipped mullet (Chelon ramada) is an important resource in the Mediterranean Sea and a candidate species for aquaculture. As microsatellite loci remain the markers of choice for the evaluation of breeding programmes or stock assessment, we hereby provide and share a resource of 3,883 species-specific microsatellite loci for this species, where they are currently lacking. This resource includes primer pairs useful for polymerase chain reaction (PCR) amplification. A subset of nine loci including di-, tri- and tetranucleotide loci with correct amplification success rates have been specifically tested under identical PCR conditions, opening up new avenues for research.