학술논문
Asperelines Produced by the Endophytic Fungus Trichoderma asperelloides From the Aquatic Plant Victoria amazonica
Document Type
Original Paper
Author
Fernandes, Kamila Rangel Primo; de Souza, Antonia Queiroz Lima; dos Santos, Lane Alencar; Nogueira, Fábio César Sousa; Evaristo, Joseph Albert Medeiros; Carneiro, Gabriel Reis Alves; da Silva, Gilvan Ferreira; da Cruz, Jeferson Chagas; Sousa, Thiago Fernandes; da Silva, Sarah Raquel Silveira; dos Santos Bastos, Ivanildes; Orlandi, Patrícia Puccinelli; de Souza, Afonso Duarte Leão
Source
Revista Brasileira de Farmacognosia: Brazilian Journal of Pharmacognosy. 31(5):667-675
Subject
Language
English
ISSN
1981-528X
Abstract
Asperelines, a family of linear peptides, produced by Trichoderma asperelloides, an endophytic strain from Victoria amazonica (Poepp.) J.C. Sowerby, Nymphaeaceae, are described. Fifteen asperelines with ten amino acid residues were identified by liquid chromatography coupled to mass spectrometry in a positive acquisition mode, using high-energy collisional dissociation. Eight of these asperelines are peptaibols of the model Ac-Aib-xxx-Val-Aib-xxx-Aib-xxx-xxx-Aib-Prolinol. The other seven have the rare proline-ending sequence type: Ac-Aib-xxx-Val-Aib-xxx-Aib-Aib-xxx-Aib-Proline. Nine new asperelines were identified, together with the known asperelines A, D, E, O, U, and Z11 . A chemotaxonomic significance of the findings and the name “peptaibes” to the aspereline types ending in amino acids are suggested. The aspereline-containing fraction showed a marginal activity against Streptococcus mutans and Staphylococcus aureus. This fraction also presented high activity against the pathogenic bacteria Listeria monocytogenes, as well as a low mortality against VERO-type cells.Graphical abstract: