학술논문
Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari)
Document Type
report
Author
Vieira, Rafael Felipe da Costa; Molento, Marcelo Beltrão; Guimarães, Ana Marcia Sá; Santos, Andrea Pires dos; Bonat, Marcelo; Javorouski, Manoel Lucas; Popp, Luciene; Santos, Leonilda Correia dos; Moraes, Wanderlei; Cubas, Zalmir Silvino; Vieira, Thállitha Samih Wischral Jayme; Vidotto, Odilon; Barros Filho, Ivan Roque; Biondo, Alexander Welker; Messick, Joanne Belle
Source
Revista Brasileira de Parasitologia Veterinária. March 2011 20(1)
Subject
Language
English
ISSN
1984-2961
Abstract
Mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR) of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari) for M. suis infection using a specific conventional PCR. A total of 28 blood samples from captive collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species.