학술논문
MOLECULAR INVESTIGATION OF HEMOTROPIC MYCOPLASMAS IN HUMAN BEINGS, DOGS AND HORSES IN A RURAL SETTLEMENT IN SOUTHERN BRAZIL
Document Type
article
Author
VIEIRA, Rafael Felipe da Costa; VIDOTTO, Odilon; VIEIRA, Thállitha Samih Wischral Jayme; GUIMARAES, Ana Márcia Sá; SANTOS, Andrea Pires dos; NASCIMENTO, Naíla Cannes; SANTOS, Nelson Jesse Rodrigues dos; MARTINS, Thiago Fernandes; LABRUNA, Marcelo Bahia; MARCONDES, Mary; BIONDO, Alexander Welker; MESSICK, Joanne Belle
Source
Revista do Instituto de Medicina Tropical de São Paulo. August 2015 57(4)
Subject
Language
English
ISSN
0036-4665
Abstract
SUMMARY The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement's population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and analyze factors associated with their infection. Blood samples from 132 dogs, 16 horses and 100 humans were screened using a pan-hemoplasma SYBR green real-time PCR assay followed by a species-specific TaqMan real-time PCR. A total of 59/132 (44.7%) dog samples were positive for hemoplasmas (21 Mycoplasma haemocanisalone, 12 ' Candidatus Mycoplasma haematoparvum' alone and 21 both). Only 1/100 (1.0%) human sample was positive by qPCR SYBR green, with no successful amplification of 16S rRNA or 23 rRNA genes despite multiple attempts. All horse samples were negative. Dogs >1 year of age were more likely to be positive for hemoplasmas ( p= 0.0014). In conclusion, although canine hemoplasma infection was highly prevalent, cross-species hemoplasma transmission was not observed, and therefore may not frequently occur despite overexposure of agents and vectors.