학술논문
Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
Document Type
article
Author
Source
Química Nova. December 2003 26(6)
Subject
Language
Portuguese
ISSN
0100-4042
Abstract
Poly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent on the same support. The highest coupling yield (59.6%) was attained when the immobilization procedure was performed at lipase loading of 150 U/g support in the presence of PEG-1.500. This immobilized system was used in esterification reactions under repeated batch cycles and the biocatalyst half-life was found to increase 2.7 times when compared with the control.