학술논문

Transferability and characterization of microsatellite markers in two Neotropical Ficus species
Document Type
article
Source
Genetics and Molecular Biology. January 2009 32(3)
Subject
ecological genetics
Ficus citrifolia
Ficus eximia
Moraceae
SSRs
Language
English
ISSN
1415-4757
Abstract
Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87.