학술논문
Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura)
Document Type
article
Source
Brazilian Journal of Genetics. December 1997 20(4)
Subject
Language
English
ISSN
0100-8455
Abstract
The a-globin major genes from diploid and tetraploid Odontophrynus americanus were studied using PCR-based technology. The cloned and sequenced amplified fragments were shown to contain most of the exon II sequences as well as the whole exon III sequence of the a-globin gene. Unexpectedly, intron 2 was entirely absent in the amplified fragments of both 2n and 4n origin. High conservation was observed among the obtained sequences when compared to corresponding sequences from human and Xenopus laevis origin. The possibility that these sequences might be pseudogenes is raised