학술논문
Type-2 diabetes alters the basal phenotype of human macrophages and diminishes their capacity to respond, internalise, and control Mycobacterium tuberculosis
Document Type
article
Author
Lopez-Lopez, Nallely; Martinez, Ana Gabriela Ramos; Garcia-Hernandez, Mariana Haydee; Hernandez-Pando, Rogelio; Castañeda-Delgado, Julio Enrique; Lugo-Villarino, Geanncarlo; Cougoule, Céline; Neyrolles, Olivier; Rivas-Santiago, Bruno; Valtierra-Alvarado, Monica Alejandra; Rubio-Caceres, Marisela; Enciso-Moreno, Jose Antonio; Serrano, Carmen Judith
Source
Memórias do Instituto Oswaldo Cruz. January 2018 113(4)
Subject
Language
English
ISSN
0074-0276
Abstract
BACKGROUND Type 2 diabetes (T2D) is a risk factor for the development of tuberculosis (TB), although the associated mechanisms are not known. OBJECTIVES To study the association between T2D and the basal phenotype of macrophages, and their immune response to Mycobacterium tuberculosis (Mtb) infection. METHODS We evaluated the influence of T2D on the response of monocyte-derived macrophages (MDM) to Mtb in patients with T2D (n = 10) compared to healthy subjects (n = 9), before and after infection with Mtb clinical isolates bearing different degrees of virulence. The levels of cell surface markers for activation secreted cytokines and chemokines, bacterial association, and intracellular bacterial growth were evaluated. FINDINGS The expression levels of HLA-DR, CD80, and CD86 were low while those of of PD-L1 were high in uninfected MDMs derived from patients with diabetes; as a result of Mtb infection, changes were only observed in the expression levels of PD-L1. The levels of cytokines (e.g., IL-6, IL-1β, IL-10, and IL-12) and chemokines (e.g., MCP-1, MIG, and RANTES) are perturbed in MDMs derived from patients with diabetes, both before infection and in response to Mtb infection. In response to the more virulent Mtb strains, the levels of association and bacterial clearance were diminished in MDMs derived from patients with diabetes. CONCLUSIONS T2D affects the basal activation state of the macrophages and its capacity to respond and control Mtb infection.