학술논문
Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle
Document Type
article
Author
Ramos, Cassiana Maria Garcez; Francisco, Julio César; Olandoski, Marcia; Carvalho, Katherine Athayde Teixeira de; Cunha, Ricardo; Erbano, Bruna Olandoski; Jorge, Lianna Ferrari; Baena, Cristina Pellegrino; Amaral, Vivian Ferreira do; Noronha, Lucia; Macedo, Rafael Michel de; Faria-Neto, José Rocha; Guarita-Souza, Luiz César
Source
Brazilian Journal of Cardiovascular Surgery. June 2014 29(2)
Subject
Language
English
ISSN
0102-7638
Abstract
Introduction: Most cardiomyocytes do not regenerate after myocardial infarction. Porcine small intestinal submucosa has been shown to be effective in tissue repair. Objective: To evaluate myocardial tissue regeneration and functional effects of SIS implantation in pigs after left ventriculotomy. Methods: Fifteen pigs were assigned to two groups: porcine small intestinal submucosa (SIS) (N=10) and control (N=5). The SIS group underwent a mini sternotomy, left ventriculotomy and placement of a SIS patch. The control group underwent a sham procedure. Echocardiography was performed before and 60 days after the surgical procedure. Histological analysis was performed with hematoxylin-eosin stain and markers for actin 1A4, anti sarcomeric actin, connexin43 and factor VIII. Results: Weight gain was similar in both groups. Echocardiography analysis revealed no difference between groups regarding end diastolic and systolic diameters and left ventricular ejection fraction, both pre (P=0.118, P=0.313, P=0.944) and post procedure (P=0.333, P=0.522, P=0.628). Both groups showed an increase in end diastolic (P<0,001 for both) and systolic diameter 60 days after surgery (P=0.005, SIS group and P=0.004, control group). New cardiomyocytes, blood vessels and inflammatory reactions were histologically identified in the SIS group. Conclusion: SIS implantation in pigs after left ventriculotomy was associated with angiomuscular regeneration and no damage in cardiac function.