학술논문
New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
Document Type
report
Author
SOUZA ROCHA, Katarine de; SCHUPP DE SENA MESQUITA, Gleiciane; SILVA FERREIRA, Maeli Fernanda; LEITE BARROS, Flávia de Nazaré; MACEDO, Renata Cecília Soares de Lima; ARAÚJO SARAIVA, Elane de; MENDES-OLIVEIRA, Ana Cristina; DUARTE CERQUEIRA, Valíria; SCOFIELD, Alessandra; GOES CAVALCANTE, Gustavo; ABEL, Isis; GUIMARÃES DE MORAES, Carla Cristina
Source
Acta Amazonica. December 2020 50(4)
Subject
Language
English
ISSN
0044-5967
Abstract
We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.