학술논문
The effect of Cratylia floribunda lectin on renal hemodynamics and ion transport
Document Type
article
Author
Havt, Alexandre; Assreuy, Ana Maria Sampaio; Nascimento, Nilberto Robson Falcão do; Fonteles, Manassés Claudino; Pereira, Lívia de Paula; Monteiro, Sandra Maria Nunes; Barbosa, Paulo Sergio Ferreira; Nascimento, Kyria Santiago do; Cavada, Benildo Sousa; Martins, Alice Maria Costa; Monteiro, Helena Serra Azul
Source
Brazilian Journal of Pharmaceutical Sciences. September 2015 51(3)
Subject
Language
English
ISSN
1984-8250
Abstract
Lectins have been described as glycoproteins that reversibly and specifically bind to carbohydrates. Legume lectins isolated from the subtribe Diocleinae (Canavalia, Dioclea andCratylia) are structurally homologous with respect to their primary structures. The Diocleinae lectins of Canavalia brasiliensis, Dioclea guianensis andCanavalia ensiformis have been shown to distinctly alter physiological parameters in isolated rat kidneys. Thus, the aim of this study was to investigate the effect of Cratylia floribunda lectin (CFL) on renal hemodynamics and ion transport in rats. In isolated perfused kidneys, CFL (10 mg/mL, n=5) increased RPP, RVR and decreased %TK+, but did not change urinary flow, glomerular filtration rate, sodium or chloride tubular transport. In isolated perfused mesenteric bed, CFL (3 and 10 mg/mL/min; n=4) did not alter tissue basal tonus or tissue contraction by phenylephrine (1 mM/mL/min). In conclusion, the seed lectin of Cratylia floribunda increased renal hemodynamic parameters showing a kaliuretic effect. This effect could be of tubular origin, rather than a result from haemodynamic alterations.