학술논문
NPY and sbGnRH gene expression in juvenile and adult male Brazilian flounder Paralichthys orbignyanus
Document Type
article
Author
Source
Ciência Rural. November 2011 41(11)
Subject
Language
English
ISSN
0103-8478
Abstract
The objective of this study was to evaluate neuropeptide Y (NPY) and sea bream gonadotropin-release hormone (sbGnRH) gene expression in juvenile and adult males of Brazilian flounder. Hypothalamuses from fish were sampled for total RNA extraction. After cDNA synthesis, real-time PCR was used to measure gene expression. NPY showed approximately 2-fold increases in their mRNA levels while sbGnRH showed 3-fold increases in adult fish. These results suggest that these peptides could be involved on hypothalamic regulation of Brazilian flounder sexual maturation.